Journal:

Article Title: Cyclin-Dependent Kinase Activity Is Required for Efficient Expression and Posttranslational Modification of Human Cytomegalovirus Proteins and for Production of Extracellular Particles

doi: 10.1128/JVI.02656-05

Figure Lengend Snippet: cdk inhibition leads to accumulation of ppUL69 in intranuclear aggregates. Human fibroblasts were infected at an MOI of 5 with HCMV Towne and seeded onto glass coverslips. At 24 (A and B) or 72 (E and F) h p.i., 20 μM Roscovitine or DMSO was added to cultures. At 72 h or 75 h p.i., cells were fixed in 2% paraformaldehyde and processed for immunofluorescent staining with antibody against ppUL69 and Hoechst stain to visualize nuclei. (C) Mock-infected control. (D) ppUL69 distribution before addition of drug or vehicle control to cultures at 72 h p.i. Images were captured with a Nikon E800 epifluorescence microscope as described in Materials and Methods. Original magnification, ×1,000.

Article Snippet: Images were captured with a Nikon E800 epifluorescence microscope as described in Materials and Methods.

Techniques: Inhibition, Infection, Staining, Microscopy

Journal:

Article Title: Cyclin-Dependent Kinase Activity Is Required for Efficient Expression and Posttranslational Modification of Human Cytomegalovirus Proteins and for Production of Extracellular Particles

doi: 10.1128/JVI.02656-05

Figure Lengend Snippet: ppUL69 is localized in aggregates around the viral replication center upon cdk inhibition. Virus-infected cells were treated with DMSO or 20 μM Roscovitine beginning at 24 h p.i. One hour prior to fixation, cells were labeled with 10 μM bromodeoxyuridine for 30 min. Cells were washed twice and refed. At 72 h p.i., cells were fixed in 2% paraformaldehyde and processed for immunofluorescent staining with antibodies against ppUL69 and BrDU (A through F). Images were captured by confocal microscopy as described in Materials and Methods. Original magnification, ×1,000. (G and H) Control stains with nonspecific rat immunoglobulin G2A (IgG2A) and mouse IgG1 antibodies, respectively. These images were captured with a Nikon E800 epifluorescence microscope as described in Materials and Methods. Original magnification, ×1,000.

Article Snippet: Images were captured with a Nikon E800 epifluorescence microscope as described in Materials and Methods.

Techniques: Inhibition, Infection, Labeling, Staining, Confocal Microscopy, Microscopy